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KMID : 1145120170150020192
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Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 2 p.192 ~ p.202
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Anti-obesity Effects of Curcuma longa L. Extracts through Inhibiting Adipogenic Transcription Factors
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Lee Seong-Ae
Ju Hyeong-Jin
Park Min-Jun
Park Bu-Duk
Kim Gyo-Nam
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Abstract
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Purpose: Curcuma longa L. (CL) has been widely used as a medicinal herb and spice. Although several biological effects, such as anti-oxidant and anti-inflammatory effects, of CL have been reported, its anti-adipogenic effects as a nutri-cosmetic material are relatively unclear. This study aimed to evaluate the anti-oxidant and anti-adipogenic effects of CL extracts (CLE) in 3T3-L1 adipocytes.
Methods: The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity of CLE was tested. Cell viability was evaluated by a thiazolyl blue tetrazolium bromide (MTT) assay. The anti-adipogenic effects of CLE were estimated using Oil Red O (ORO) staining in 3T3-L1 adipocytes. In addition, number and size of lipid droplets were determined by photomicrograph analysis. The mRNA expression levels of adipogenic transcriptional factors in CLE-treated 3T3-L1 adipocytes were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).
Results: Up to 10 mg/mL concentration of CLE effectively scavenged DPPH radicals. Treatment with up to 800 μg/mL concentration of CLE for 24 h did not affect 3T3-L1 adipocytes viability. Thus, we chose up to 800 μg/mL CLE for further experiments. Treatment with CLE significantly inhibited lipid accumulation, and decreased number and size of lipid droplets in 3T3-L1 adipocytes. In addition, down-regulated CCAAT/enhancer binding protein β (CEBPB), peroxisome proliferator activated receptor γ (PPARG), CCAAT/enhancer binding protein α (CEBPA), fatty acid synthase (FASN), and lipoprotein lipase (LPL) expression levels were observed in CLE treated 3T3-L1 cell.
Conclusion: Our results suggest that CLE could be used as a potential nutri-cosmetic material that exhibits anti-oxidant and anti-adipogenic effects.
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KEYWORD
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Curcuma longa L., Obesity, Adipogenesis, Anti-oxidant effects, Oxidative stress
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